KETAHANAN BENIH IKAN NILA GIFT (Oreochromis niloticus LINN.) DARI HASIL INDUK YANG DIBERI VAKSIN TERHADAP INFEKSI BUATAN Streptococcus iniae Resistance of Fry from Vaccinated Mother of Gift Tilapia {Oreochromis niloticus Linn.) to Artificial Infection of Streptococcus iniae
نویسنده
چکیده
An investigation was made to study the efficacy of dosage and time of administration in maternal vaccination. Mothers of nile tilapia were vaccinated by intraperitoneal injection with adjuvant-heat killed Streptococcus iniae at one and two weeks after spawning at the dosages of 0,2 ml/kg and 0,4 ml/kg body weight. Unvaccinated mothers were used as control. Agglutinating antibody titers in the blood plasma of mothers before and after spawning, eggs soluble extract, the body fluid of fry at 5, 10 and 15 days post hatching (DPH) were examined. The protective immunity of fry was tested by challenge test, the survival rate (SR) and the relative percent survival (RPS) of fish within 7 days was observed. There was no difference in the antibody level of mothers, eggs soluble extract, and the body fluid of fry at 5, 10 and 15 DPH from vaccinated mothers at one or two weeks after spawning. However, antibody of mothers, eggs and fry from vaccinated mothers at the dosage of 0,4 ml/kg body weight were higher than the dosage of 0,2 ml/kg body weight and control. Antibody titre.of fry of vaccinated mothers at 5. 10 and 15 DPH were (-log2=2,88), (-log2=2,53) and (-log2=2,07) respectively, while SR were 89%, 94% and 92% respectively. SR of control fry were lower 7%, 10% and 12% respectively than fry from vaccinated mother; meanwhile RPS were 47%, 76% and 77% respectively.
منابع مشابه
Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus).
A novel attenuated Streptococcus iniae vaccine was developed from a virulent strain of Streptococcus iniae (ISET0901) through selection for novobiocin resistance (named ISNO). The safety of ISNO was then evaluated in Nile tilapia (Oreochromis niloticus) through intraperitoneal (IP) injection. When male tilapia (average weight 10 g) were IP injected with 2×10(7) colony-forming units (CFU) of the...
متن کاملComplete Genome Sequence of Streptococcus iniae UEL-Si1, Isolated in Diseased Nile Tilapia (Oreochromis niloticus) from Northern Paraná, Southern Brazil
The Streptococcus iniae UEL-Si1 strain was isolated from diseased Nile tilapia within the Paranapanema River Basin, Northern Paraná, Brazil. This is an emerging infectious disease agent of fish from Brazil, and sequencing of the complete genome is fundamental to understanding aspects relative to pathogenesis, infection, epidemiology, and immunity.
متن کاملEnhanced mortality in Nile tilapia Oreochromis niloticus following coinfections with ichthyophthiriasis and streptococcosis.
Ichthyophthirius multifiliis Fouquet (Ich) and Streptococcus iniae are 2 major pathogens of cultured Nile tilapia Oreochromis niloticus (L). Currently there is no information available for the effect of coinfection by Ich and S. iniae on fish. The objective of this study was to determine the effects of parasite load and Ich development size on fish mortality following S. iniae infection. Low mo...
متن کاملImmunoproteomic analysis of the antibody response obtained in Nile tilapia following vaccination with a Streptococcus iniae vaccine.
Streptococcus iniae is one of the most economically important Gram-positive pathogens in cultured fish species worldwide. The USDA-ARS Aquatic Animal Health Research Unit developed a modified (contains concentrated culture supernatant) S. iniae bacterin that has been demonstrated to be efficacious, and protection is mediated by specific anti-S. iniae antibodies. Although effective, the specific...
متن کامل